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What is a T7 promoter?
The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5′ – TAATACGACTCACTATAG – 3′) that is recognized by T7 RNA polymerase1. Click to see full answer Also question is, how does t7 promoter work?
Can I use promoter to incubate T7 RNA polymerase?
Promoter for bacteriophage T7 RNA polymerase. Sticky ends from different SfcI sites may not be compatible. SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations. Sticky ends from different SfcI sites may not be compatible.
What is the role of the T7 RNA polymerase during heat induction?
Upon heat induction, the T7 RNA polymerase is produced and initiates transcription on the expression vector, resulting in turn in the expression of the gene (s) under the control of p (T7).
What is a T7 vector used for?
The T7 expression vectors are designed to facilitate cloning using Gateway® technology, and easy protein purification and detection. What does t7 RNA polymerase do? T7 RNA polymerase.
What are the characteristics of T7 polymerase?
Activity. T7 polymerase is extremely promoter-specific and transcribes only DNA downstream of a T7 promoter. The T7 polymerase also requires a double stranded DNA template and Mg 2+ ion as cofactor for the synthesis of RNA. It has a very low error rate. T7 polymerase has a molecular weight of 99 kDa. Promoter
Where does T7 RNA polymerase start transcription?
T7 RNA polymerase starts transcription at the underlined G in the promoter sequence. The polymerase then transcribes using the opposite strand as a template from 5’->3’. The first base in the transcript will be a G.
What is a T7 expression vector?
The T7 expression system allows high-level expression from the strong bacteriophage T7 promoter. It’s ideal for expressing soluble, nontoxic recombinant proteins in E. coli. The T7 expression vectors are designed to facilitate cloning using Gateway® technology, and easy protein purification and detection. What does t7 RNA polymerase do?